Journal: Analytical and Bioanalytical Chemistry
Article Title: Development of a GC-MS/MS method to quantify 120 gut microbiota-derived metabolites
doi: 10.1007/s00216-025-06256-6
Figure Lengend Snippet: Metabolites quantified in cecum and plasma of CONV and GF mice analyzed in different measurement batches to assess intra- and interday measurement precision and metabolites quantified in cecal samples of conventional animals stored under three different preanalytical conditions. a Box plots of citric acid and indole-propionic acid quantified in the plasma of CONV and GF animals, and butyric acid and glycine quantified in the cecum of CONV and GF animals. The same samples were processed in three independent batches (each containing four identical replicates) on three different days, and each processed batch was measured by GC-MS/MS three times (two times on the same day and once on the subsequent day). b Scatter plot of quantified metabolites in cecum sample stored in Invitek and OMNIgut stabilization buffers and in a freshly frozen sample. The abundances of all 82 quantified metabolites are represented as mean concentrations from four replicates in each group.
Article Snippet: Chromatographic data was acquired and processed using Shimadzu LabSolutions GC-MS software (version 5.131, Shimadzu Corporation, Kyoto, Japan).
Techniques: Clinical Proteomics, Gas Chromatography-Mass Spectrometry